Unique Immune Gene Expression Patterns in Bronchoalveolar Lavage and Tumor Adjacent Non-Neoplastic Lung Tissue in Non-Small Cell Lung Cancer.

ABSTRACT:

Background

The immune cells in the local environments surrounding non-small cell lung cancer (NSCLC) implicate the balance of pro- and antitumor immunity; however, their transcriptomic profiles remain poorly understood.

Methods

A transcriptomic microarray study of bronchoalveolar lavage (BAL) cells harvested from tumor-bearing lung segments was performed in a discovery group. The findings were validated (1) in published microarray datasets, (2) in an independent group by RT-qPCR, and (3) in non-diseased and tumor adjacent non-neoplastic lung tissue by immunohistochemistry and in BAL cell lysates by immunoblotting.

Result

The differential expression of 129 genes was identified in the discovery group. These genes revealed functional enrichment in Fc gamma receptor-depen

SUBMITTER: Kuo CS 

PROVIDER: S-EPMC5816075 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature