Morphology-driven downscaling of Streptomyces lividans to micro-cultivation.

ABSTRACT: Actinobacteria are prolific producers of secondary metabolites and industrially relevant enzymes. Growth of these mycelial micro-organisms in small culture volumes is challenging due to their complex morphology. Since morphology and production are typically linked, scaling down culture volumes requires better control over morphogenesis. In larger scale platforms, ranging from shake flasks to bioreactors, the hydrodynamics play an important role in shaping the morphology and determining product formation. Here, we report on the effects of agitation on the mycelial morphology of Streptomyces lividans grown in microtitre plates. Our work shows that at the appropriate agitation rates cultures can be scaled down to volumes as small as 100 µl while maintaining the same morphology as seen in larg

SUBMITTER: van Dissel D 

PROVIDER: S-EPMC5816114 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature