Supplementation of L-carnitine during in vitro maturation of mouse oocytes affects expression of genes involved in oocyte and embryo competence: An experimental study.

ABSTRACT:

Background

Oocyte developmental competence is one of the key factors for determining the success rate of assisted reproductive technique.

Objective

The aim of the current study was to investigate the effect of L-carnitine (LC) supplementation during in vitro maturation (IVM), on preimplantation embryo development and expression of genes involved in embryo competence derived from oocytes selected with brilliant cresyl blue (BCB) test.

Materials and methods

Cumulus-oocyte complexes (COCs) were obtained from NMRI mice ovaries. COCs were stained with BCB and then BCB+ (colored cytoplasm) oocytes cultured in IVM medium supplemented with 0.3 or 0.6 mg/ml LC. COCs untreated with LC were used as control. Fertilization rate and blastocyst development rate were determined after

SUBMITTER: Zare Z 

PROVIDER: S-EPMC5816238 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature