In vitro and in vivo characterization of three <i>Cellvibrio japonicus</i> glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions.

ABSTRACT:

Background

Xyloglucan (XyG) is a ubiquitous and fundamental polysaccharide of plant cell walls. Due to its structural complexity, XyG requires a combination of backbone-cleaving and sidechain-debranching enzymes for complete deconstruction into its component monosaccharides. The soil saprophyte Cellvibrio japonicus has emerged as a genetically tractable model system to study biomass saccharification, in part due to its innate capacity to utilize a wide range of plant polysaccharides for growth. Whereas the downstream debranching enzymes of the xyloglucan utilization system of C. japonicus have been functionally characterized, the requisite backbone-cleaving endo-xyloglucanases were unresolved.

Results

Combined bioinformatic and transcriptomic analyses im

SUBMITTER: Attia MA 

PROVIDER: S-EPMC5816542 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature