The transcriptome response of the ruminal methanogen Methanobrevibacter ruminantium strain M1 to the inhibitor lauric acid.

ABSTRACT:

Objective

Lauric acid (C₁₂) is a medium-chain fatty acid that inhibits growth and production of the greenhouse gas methane by rumen methanogens such as Methanobrevibacter ruminantium. To understand the inhibitory mechanism of C₁₂, a transcriptome analysis was performed in M. ruminantium strain M1 (DSM 1093) using RNA-Seq.

Results

Pure cell cultures in the exponential growth phase were treated with 0.4 mg/ml C₁₂, dissolved in dimethyl sulfoxide (DMSO), for 1 h and transcriptomic changes were compared to DMSO-only treated cells (final DMSO concentration 0.2%). Exposure to C₁₂ resulted in differential expression of 163 of the 2280 genes in the M1 genome (maximum log₂-fold change 6.6). Remarkably, C₁₂ hardly affec

SUBMITTER: Zhou X 

PROVIDER: S-EPMC5816558 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature