Hospital Epidemiology of Methicillin-Resistant Staphylococcus aureus in a Tertiary Care Hospital in Moshi, Tanzania, as Determined by Whole Genome Sequencing.

ABSTRACT:

Objective

To determine molecular epidemiology of methicillin-resistant S. aureus in Tanzania using whole genome sequencing.

Methods

DNA from 33 Staphylococcus species was recovered from subcultured archived Staphylococcus isolates. Whole genome sequencing was performed on Illumina Miseq using paired-end 2 × 250 bp protocol. Raw sequence data were analyzed using online tools.

Results

Full susceptibility to vancomycin and chloramphenicol was observed. Thirteen isolates (43.3%) resisted cefoxitin and other antimicrobials tested. Multilocus sequence typing revealed 13 different sequence types among the 30 S. aureus isolates, with ST-8 (n = seven, 23%) being the most common. Gene detection in S. aureus stains were as follows

SUBMITTER: Kumburu HH 

PROVIDER: S-EPMC5816877 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature