Regulating NETosis: Increasing pH Promotes NADPH Oxidase-Dependent NETosis.

ABSTRACT: Neutrophils migrating from the blood (pH 7.35-7.45) into the surrounding tissues encounter changes in extracellular pH (pH_e) conditions. Upon activation of NADPH oxidase 2 (Nox), neutrophils generate large amounts of H⁺ ions reducing the intracellular pH (pH_i). Nevertheless, how extracellular pH regulates neutrophil extracellular trap (NET) formation (NETosis) is not clearly established. We hypothesized that increasing pH increases Nox-mediated production of reactive oxygen species (ROS) and neutrophil protease activity, stimulating NETosis. Here, we found that raising pH_e (ranging from 6.6 to 7.8; every 0.2 units) increased pH_i of both activated and resting neutrophils within 10-20 min (Seminaphtharhodafluor dual fluorescence measurem

SUBMITTER: Khan MA 

PROVIDER: S-EPMC5816902 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature