²¹²Pb-labeled B7-H3-targeting antibody for pancreatic cancer therapy in mouse models.

ABSTRACT:

Introduction

We recently validated monoclonal antibody (mAb) 376.96 as an effective carrier for targeted α-particle radioimmunotherapy (RIT) with ²¹²Pb in ovarian cancer mouse models. In this study, we tested the binding of radiolabeled mAb 376.96 to human pancreatic ductal adenocarcinoma (PDAC) cells and localization in xenografts in immune-deficient mice and evaluated ²¹²Pb-labeled 376.96 (²¹²Pb-376.96) for PDAC therapy.

Methods

In vitro Scatchard assays assessed the specific binding of ²¹²Pb-376.96 to human PDAC3 adherent differentiated cells and non-adherent cancer initiating cells (CICs) dissociated from tumorspheres. In vitro clonogenic assays were used to measure the proliferation of adherent PDAC3 cells and CIC-enriched tum

SUBMITTER: Kasten BB 

PROVIDER: S-EPMC5817013 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature