Prevalence of polyreactive innate clones among graft--infiltrating B cells in human cardiac allograft vasculopathy.

ABSTRACT:

Background

Cardiac allograft vasculopathy (CAV) has been associated with graft-infiltrating B cells, although their characteristics are still unclear. In this study we examined the frequency, localization and reactivity profile of graft-infiltrating B cells to determine their contribution to the pathophysiology of CAV.

Methods

B cells, plasma cells and macrophages were examined by immunohistochemistry in 56 allografts with CAV, 49 native failed hearts and 25 autopsy specimens. A total of 102 B-cell clones were immortalized directly from the infiltrates of 3 fresh cardiac samples with CAV. Their secreted antibodies were assessed using enzyme-linked immunoassay and flow cytometry.

Results

B-cell infiltration was observed around coronary arteries in 93% of allograft expl

SUBMITTER: Chatterjee D 

PROVIDER: S-EPMC5817022 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature