Construction of chloroplast transformation vector and its functional evaluation in Momordica charantia L.

ABSTRACT: Chloroplast transformation vectors require an expression cassette flanked by homologous plastid sequences to drive plastome recombination. The rrn16-rrn23 plastome region was selected and using this region, a new species-specific plastid transformation vector CuIA was developed with pKS⁺II as a backbone by inserting the rrn16-trnI and trnA-rrn23 sequences from Cucumis sativus L. An independent expression cassette with aadA gene encoding aminoglycoside 3'-adenylyltransferase with psbA controlling elements is added into the trnI-trnA intergenic region that confers resistance to spectinomycin. An efficient plastid transformation in bitter melon (Momordica charantia L.) was achieved by bombardment of

SUBMITTER: Narra M 

PROVIDER: S-EPMC5817051 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature