The rate of lactate production from glucose in hearts is not altered by per-deuteration of glucose.

ABSTRACT: This study was designed to determine whether perdeuterated glucose experiences a kinetic isotope effect (KIE) as glucose passes through glycolysis and is further oxidized in the tricarboxylic acid (TCA) cycle. Metabolism of deuterated glucose was investigated in two groups of perfused rat hearts. The control group was supplied with a 1:1 mixture of [U-¹³C₆]glucose and [1,6-¹³C₂]glucose, while the experimental group received [U-¹³C₆,U-²H₇]glucose and [1,6-¹³C₂]glucose. Tissue extracts were analyzed by ¹H, ²H and proton-decoupled ¹³C NMR spectroscopy. Extensive ²H-¹³C scalar coupling plus chemical shift isotope effects were obser

SUBMITTER: Funk AM 

PROVIDER: S-EPMC5817885 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature