Molecular Identification of Bacteria in Intra-abdominal Abscesses Using Deep Sequencing.

ABSTRACT:

Background

Intra-abdominal abscesses are localized collections of pus, which generally arise from a breach in the normal mucosal defense barrier that allows bacteria from gastrointestinal tract, and less commonly from the gynecologic or urinary tract, to induce inflammation, resulting in an infection. The microbiology of these abscesses is usually polymicrobial, associated with the primary disease process. However, the microbial identity, diversity and richness in intra-abdominal abscesses have not been well characterized, due in part to the difficulty in cultivating commensal organisms using standard culture-based techniques.

Methods

We used culture-independent 16S rRNA Illumina sequencing to characterize bacterial communities in intra-abdominal abscesses collected by percu

SUBMITTER: Kozlov A 

PROVIDER: S-EPMC5817941 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature