Metabolic heterogeneity and plasticity of glioma stem cells in a mouse glioblastoma model.

ABSTRACT:

Background

Glioblastomas have been shown to rely on glycolysis as an energy source. However, recent evidence suggests that at least a subset of glioma cells with stem cell-like properties can thrive on oxidative phosphorylation. It remains unclear whether both metabolic phenotypes support tumor propagation, if they are independent, and how stable they are. The present study investigated these questions with the use of isogenic murine glioma stem cells (GSCs).

Methods

GSCs were established from tumors formed by Ink4a/Arf-null, H-RasV12-expressing glioma-initiating cells that differed in extracellular acidification potential. Metabolic characteristics of GSCs were determined by measurement of glucose, oxygen, and glutamine uptake, ATP content, and lactate production. Effects o

SUBMITTER: Shibao S 

PROVIDER: S-EPMC5817950 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature