Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human pre-implantation epiblast.

ABSTRACT: Single-cell profiling techniques create opportunities to delineate cell fate progression in mammalian development. Recent studies have provided transcriptome data from human pre-implantation embryos, in total comprising nearly 2000 individual cells. Interpretation of these data is confounded by biological factors, such as variable embryo staging and cell-type ambiguity, as well as technical challenges in the collective analysis of datasets produced with different sample preparation and sequencing protocols. Here, we address these issues to assemble a complete gene expression time course spanning human pre-implantation embryogenesis. We identify key transcriptional features over developmental time and elucidate lineage-specific regulatory networks. We resolve post-hoc cell-type assignment i

SUBMITTER: Stirparo GG 

PROVIDER: S-EPMC5818005 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature