Substitution of the D1-Asn⁸⁷ site in photosystem II of cyanobacteria mimics the chloride-binding characteristics of spinach photosystem II.

ABSTRACT: Photoinduced water oxidation at the O₂-evolving complex (OEC) of photosystem II (PSII) is a complex process involving a tetramanganese-calcium cluster that is surrounded by a hydrogen-bonded network of water molecules, chloride ions, and amino acid residues. Although the structure of the OEC has remained conserved over eons of evolution, significant differences in the chloride-binding characteristics exist between cyanobacteria and higher plants. An analysis of amino acid residues in and around the OEC has identified residue 87 in the D1 subunit as the only significant difference between PSII in cyanobacteria and higher plants. We substituted the D1-Asn⁸⁷ residue in the cyanobacterium Synechocystis sp. PCC 6803 (wildtype) with alanine, present in higher plants,

SUBMITTER: Banerjee G 

PROVIDER: S-EPMC5818196 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature