New penta-saccharide-bearing tripod amphiphiles for membrane protein structure studies.

ABSTRACT: Integral membrane proteins either alone or as complexes carry out a range of key cellular functions. Detergents are indispensable tools in the isolation of membrane proteins from biological membranes for downstream studies. Although a large number of techniques and tools, including a wide variety of detergents, are available, purification and structural characterization of many membrane proteins remain challenging. In the current study, a new class of tripod amphiphiles bearing two different penta-saccharide head groups, designated TPSs, were developed and evaluated for their ability to extract and stabilize a range of diverse membrane proteins. Variations in the structures of the detergent head and tail groups allowed us to prepare three sets of the novel agents with distinctive structure

SUBMITTER: Ehsan M 

PROVIDER: S-EPMC5818263 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature