The Ca_V2α1 EF-hand F helix tyrosine, a highly conserved locus for GPCR inhibition of Ca_V2 channels.

ABSTRACT: The sensory neuron of Aplysia californica participates in several forms of presynaptic plasticity including homosynaptic depression, heterosynaptic depression, facilitation and the reversal of depression. The calcium channel triggering neurotransmitter release at most synapses is Ca_V2, consisting of the pore forming α1 subunit (Ca_V2α1), and auxiliary Ca_Vβ, and Ca_Vα2δ subunits. To determine the role of the Ca_V2 channel in presynaptic plasticity in Aplysia, we cloned Aplysia Ca_V2α1, Ca_Vβ, and Ca_Vα2δ and over-expressed the proteins in Aplysia sensory neurons (SN). We show expression of exogenous Ca_V2α1 in the neurites of cultured Aplysia SN. One proposed mechanism for heterosynaptic depression i

SUBMITTER: Dunn TW 

PROVIDER: S-EPMC5818475 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature