Mesenchyme-derived factors enhance preneoplastic growth by non-genotoxic carcinogens in rat liver.

ABSTRACT: Many frequently prescribed drugs are non-genotoxic carcinogens (NGC) in rodent liver. Their mode of action and health risks for humans remain to be elucidated. Here, we investigated the impact of two model NGC, the anti-epileptic drug phenobarbital (PB) and the contraceptive cyproterone acetate (CPA), on intrahepatic epithelial-mesenchymal crosstalk and on growth of first stages of hepatocarcinogenesis. Unaltered hepatocytes (HC) and preneoplastic HC (HC_PREN) were isolated from rat liver for primary culture. DNA replication of HC and HC_PREN was increased by in vitro treatment with 10 µM CPA, but not 1 mM PB. Next, mesenchymal cells (MC) obtained from liver of rats treated with either PB (50 mg/kg bw/day) or CPA (100 mg/kg bw/day), were cultured. Supernatants from both

SUBMITTER: Nejabat M 

PROVIDER: S-EPMC5818586 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature