Roles of cytosolic phospholipase A₂α in reproductive and systemic toxicities in 2,3,7,8-tetrachlorodibenzo-p-dioxin-exposed mice.

ABSTRACT: Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces a variety of toxicities upon binding of TCDD to aryl hydrocarbon receptor. Although this binding upregulates the synthesis of prostaglandins and their related lipid mediators via cytosolic phospholipase A₂α (cPLA₂α), toxicological significance of this signaling pathway remains elusive. Herein, we investigated the roles of cPLA₂α in TCDD toxicities using cPLA₂α-null mice. In a first set of experiments, pregnant mice were orally administered TCDD at a dose of 40 μg/kg on gestation day (GD) 12.5, and fetuses were collected on GD 18 for subsequent analyses. The number of live male fetuses of cPLA₂α-null type was significantly less than that of wild-type in TCDD-exposed litters.

SUBMITTER: Fujisawa N 

PROVIDER: S-EPMC5818604 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature