A single NaK channel conformation is not enough for non-selective ion conduction.

ABSTRACT: NaK and other non-selective channels are able to conduct both sodium (Na⁺) and potassium (K⁺) with equally high efficiency. In contrast to previous crystallographic results, we show that the selectivity filter (SF) of NaK in native-like lipid membranes adopts two distinct conformations that are stabilized by either Na⁺ or K⁺ ions. The atomic differences of these conformations are resolved by solid-state NMR (ssNMR) spectroscopy and molecular dynamics (MD) simulations. Besides the canonical K⁺ permeation pathway, we identify a side entry ion-conduction pathway for Na⁺ permeation unique to NaK. Moreover, under otherwise identical conditions ssNMR spectra of the K⁺ selective NaK mutant (NaK2K) reveal only a single co

SUBMITTER: Shi C 

PROVIDER: S-EPMC5818664 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature