Molecular characterization of human Dirofilaria isolates from Kerala.

ABSTRACT:

Background & objectives

Human dirofilariosis is a well-recognized zoonosis caused by several species of the genus Dirofilaria. The disease is prevalent among canines and human beings in Kerala. The objective of the present study was to confirm the human Dirofilaria isolates by molecular characterization.

Methods

The worms or segments obtained from human sources were subjected to diagnostic polymerase chain reaction (PCR) targeting the cytochrome oxidase subunit 1 (COI) and 5S ribosomal RNA (rRNA) genes of Dirofilaria repens. The amplicons were sequenced and analyzed.

Results

The filariid nematodes recovered from ocular as well as subcutaneous tissue of human origin were identified as D. repens based on PCR targeting COI as well as 5S rRNA genes. The phylogenetic analy

SUBMITTER: Nazar N 

PROVIDER: S-EPMC5819036 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature