Dynamics of promoter bivalency and RNAP II pausing in mouse stem and differentiated cells.

ABSTRACT:

Background

Mammalian embryonic stem cells display a unique epigenetic and transcriptional state to facilitate pluripotency by maintaining lineage-specification genes in a poised state. Two epigenetic and transcription processes involved in maintaining poised state are bivalent chromatin, characterized by the simultaneous presence of activating and repressive histone methylation marks, and RNA polymerase II (RNAPII) promoter proximal pausing. However, the dynamics of histone modifications and RNAPII at promoters in diverse cellular contexts remains underexplored.

Results

We collected genome wide data for bivalent chromatin marks H3K4me3 and H3K27me3, and RNAPII (8WG16) occupancy together with expression profiling in eight different cell types, including ESCs, in mouse. The ep

SUBMITTER: Mantsoki A 

PROVIDER: S-EPMC5819258 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature