Redirection of lipid flux toward phospholipids in yeast increases fatty acid turnover and secretion.

ABSTRACT: Bio-based production of fatty acids and fatty acid-derived products can enable sustainable substitution of petroleum-derived fuels and chemicals. However, developing new microbial cell factories for producing high levels of fatty acids requires extensive engineering of lipid metabolism, a complex and tightly regulated metabolic network. Here we generated a Saccharomyces cerevisiae platform strain with a simplified lipid metabolism network with high-level production of free fatty acids (FFAs) due to redirected fatty acid metabolism and reduced feedback regulation. Deletion of the main fatty acid activation genes (the first step in β-oxidation), main storage lipid formation genes, and phosphatidate phosphatase genes resulted in a constrained lipid metabolic network in which fatty acid

SUBMITTER: Ferreira R 

PROVIDER: S-EPMC5819412 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature