Finding RNA structure in the unstructured RBPome.

ABSTRACT:

Background

RNA-binding proteins (RBPs) play vital roles in many processes in the cell. Different RBPs bind RNA with different sequence and structure specificities. While sequence specificities for a large set of 205 RBPs have been reported through the RNAcompete compendium, structure specificities are known for only a small fraction. The main limitation lies in the design of the RNAcompete technology, which tests RBP binding against unstructured RNA probes, making it difficult to infer structural preferences from these data. We recently developed RCK, an algorithm to infer sequence and structural binding models from RNAcompete data. The set of binding models enables, for the first time, a large-scale assessment of RNA structure in the RBPome.

Results

We re-validate and uncov

SUBMITTER: Orenstein Y 

PROVIDER: S-EPMC5819699 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature