Induction of the Spx regulon by cell wall stress reveals novel regulatory mechanisms in Bacillus subtilis.

ABSTRACT: The transcription factor Spx is the master regulator of the disulfide stress response in Bacillus subtilis. Intriguingly, the activation of Spx by diamide relies entirely on posttranslational regulatory events in spite of the complex transcriptional control of the spx gene. Here, we show that cell wall stress, but not membrane stress, also results in induction of the Spx regulon. Remarkably, two major differences were found regarding the mechanism of induction of Spx under cell wall stress in comparison to disulfide stress. First, transcriptional induction of the spx gene from a σ^M -dependent promoter is required for accumulation of Spx in response to cell wall stress. Second, activation of the Spx regulon during cell wall stress is not accompanied by oxidation of the Spx disulf

SUBMITTER: Rojas-Tapias DF 

PROVIDER: S-EPMC5820111 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature