Identification of interfaces involved in weak interactions with application to F-actin-aldolase rafts.

ABSTRACT: Macromolecular interactions occur with widely varying affinities. Strong interactions form well defined interfaces but weak interactions are more dynamic and variable. Weak interactions can collectively lead to large structures such as microvilli via cooperativity and are often the precursors of much stronger interactions, e.g. the initial actin-myosin interaction during muscle contraction. Electron tomography combined with subvolume alignment and classification is an ideal method for the study of weak interactions because a 3-D image is obtained for the individual interactions, which subsequently are characterized collectively. Here we describe a method to characterize heterogeneous F-actin-aldolase interactions in 2-D rafts using electron tomography. By forming separate averages of the t

SUBMITTER: Hu G 

PROVIDER: S-EPMC5820182 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature