Orthogonality and Burdens of Heterologous AND Gate Gene Circuits in E. coli.

ABSTRACT: Synthetic biology approaches commonly introduce heterologous gene networks into a host to predictably program cells, with the expectation of the synthetic network being orthogonal to the host background. However, introduced circuits may interfere with the host's physiology, either indirectly by posing a metabolic burden and/or through unintended direct interactions between parts of the circuit with those of the host, affecting functionality. Here we used RNA-Seq transcriptome analysis to quantify the interactions between a representative heterologous AND gate circuit and the host Escherichia coli under various conditions including circuit designs and plasmid copy numbers. We show that the circuit plasmid copy number outweighs circuit composition for their effect on host gene expression wit

SUBMITTER: Liu Q 

PROVIDER: S-EPMC5820654 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature