Slowdown of Translational Elongation in Escherichia coli under Hyperosmotic Stress.

ABSTRACT: In nature, bacteria frequently experience many adverse conditions, including heat, oxidation, acidity, and hyperosmolarity, which all tend to slow down if not outright stop cell growth. Previous work on bacterial stress mainly focused on understanding gene regulatory responses. Much less is known about how stresses compromise protein synthesis, which is the major driver of cell growth. Here, we quantitatively characterize the translational capacity of Escherichia coli cells growing exponentially under hyperosmotic stress. We found that hyperosmotic stress affects bacterial protein synthesis through reduction of the translational elongation rate, which is largely compensated for by an increase in the cellular ribosome content compared with nutrient limitation at a similar growth rate. The s

SUBMITTER: Dai X 

PROVIDER: S-EPMC5821080 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature