Differences between co-cultures and monocultures in testing the toxicity of particulate matter derived from log wood and pellet combustion.

ABSTRACT:

Background

In vitro studies with monocultures of human alveolar cells shed deeper knowledge on the cellular mechanisms by which particulate matter (PM) causes toxicity, but cannot account for mitigating or aggravating effects of cell-cell interactions on PM toxicity.

Methods

We assessed inflammation, oxidative stress as well as cytotoxic and genotoxic effects induced by PM from the combustion of different types of wood logs and softwood pellets in three cell culture setups: two monocultures of either human macrophage-like cells or human alveolar epithelial cells, and a co-culture of these two cell lines. The adverse effects of the PM samples were compared between these setups.

Results

We detected clear differences in the endpoints between the mono- and co-cultures. In

SUBMITTER: Kasurinen S 

PROVIDER: S-EPMC5821343 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature