Self-organization of human embryonic stem cells on micropatterns.

ABSTRACT: Fate allocation in the gastrulating embryo is spatially organized as cells differentiate into specialized cell types depending on their positions with respect to the body axes. There is a need for in vitro protocols that allow the study of spatial organization associated with this developmental transition. Although embryoid bodies and organoids can exhibit some spatial organization of differentiated cells, methods that generate embryoid bodies or organoids do not yield consistent and fully reproducible results. Here, we describe a micropatterning approach in which human embryonic stem cells are confined to disk-shaped, submillimeter colonies. After 42 h of BMP4 stimulation, cells form self-organized differentiation patterns in concentric radial domains, which express specific markers assoc

SUBMITTER: Deglincerti A 

PROVIDER: S-EPMC5821517 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature