Bone biopsy protocol for advanced prostate cancer in the era of precision medicine.

ABSTRACT: BACKGROUND:Metastatic biopsies are increasingly being performed in patients with advanced prostate cancer to search for actionable targets and/or to identify emerging resistance mechanisms. Due to a predominance of bone metastases and their sclerotic nature, obtaining sufficient tissue for clinical and genomic studies is challenging. METHODS:Patients with prostate cancer bone metastases were enrolled between February 2013 and March 2017 on an institutional review board-approved protocol for prospective image-guided bone biopsy. Bone biopsies and blood clots were collected fresh. Compact bone was subjected to formalin with a decalcifying agent for diagnosis; bone marrow and blood clots were frozen in optimum cutting temperature formulation for next-generation sequencing. Frozen slides were

SUBMITTER: Sailer V 

PROVIDER: S-EPMC5821525 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature