Mechanistic target of rapamycin complex 1 and 2 in human temporal lobe epilepsy.

ABSTRACT:

Objective

Temporal lobe epilepsy (TLE) is a chronic epilepsy syndrome defined by seizures and progressive neurological disabilities, including cognitive impairments, anxiety, and depression. Here, human TLE specimens were investigated focusing on the mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) and complex 2 (mTORC2) activities in the brain, given that both pathways may represent unique targets for treatment.

Methods

Surgically resected hippocampal and temporal lobe samples from therapy-resistant TLE patients were analyzed by western blotting to quantify the expression of established mTORC1 and mTORC2 activity markers and upstream or downstream signaling pathways involving the two complexes. Histological and immunohistochemical techniques were used to assess hip

SUBMITTER: Talos DM 

PROVIDER: S-EPMC5821556 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature