Cytokine- and TCR-Mediated Regulation of T Cell Expression of Ly6C and Sca-1.

ABSTRACT: Ly6C and Sca-1 (Ly6A/E) are Ly6 family GPI-anchored surface molecules that are differentially expressed by multiple immune populations. Ly6C expression has been used to distinguish short-lived effector CD4⁺ T cells from memory precursor effector cells, whereas Sca-1 has been used in the identification of CD8⁺ memory stem cells. This study examines the expression patterns of these molecules and establishes that, in vitro, IL-27, type I IFN, and IFN-γ are potent inducers of Ly6C and Sca-1 in naive mouse CD4⁺ and CD8⁺ T cells, whereas TGF-β limits their expression. The induction of Ly6C and Sca-1 by IL-27 and IFN-γ is dependent on STAT1, but not STAT3 or T-bet. In mouse splenocytes, at homeostasis, Ly6C and Sca-1 expression was not restricted to eff

SUBMITTER: DeLong JH 

PROVIDER: S-EPMC5821564 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature