Probing cardiac metabolism by hyperpolarized 13C MR using an exclusively endogenous substrate mixture and photo-induced nonpersistent radicals.

ABSTRACT:

Purpose

To probe the cardiac metabolism of carbohydrates and short chain fatty acids simultaneously in vivo following the injection of a hyperpolarized ¹³ C-labeled substrate mixture prepared using photo-induced nonpersistent radicals.

Methods

Droplets of mixed [1-¹³ C]pyruvic and [1-¹³ C]butyric acids were frozen into glassy beads in liquid nitrogen. Ethanol addition was investigated as a means to increase the polarization level. The beads were irradiated with ultraviolet light and the radical concentration was measured by ESR spectroscopy. Following dynamic nuclear polarization in a 7T polarizer, the beads were dissolved, and the radical-free hyperpolarized solution was rapidly transferred into an injection pump located inside a 9.4T scanne

SUBMITTER: Bastiaansen JAM 

PROVIDER: S-EPMC5821575 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature