Comparative transcriptomics indicate changes in cell wall organization and stress response in seedlings during spaceflight.

ABSTRACT:

Premise of the study

Plants will play an important role in the future of space exploration as part of bioregenerative life support. Thus, it is important to understand the effects of microgravity and spaceflight on gene expression in plant development.

Methods

We analyzed the transcriptome of Arabidopsis thaliana using the Biological Research in Canisters (BRIC) hardware during Space Shuttle mission STS-131. The bioinformatics methods used included RMA (robust multi-array average), MAS5 (Microarray Suite 5.0), and PLIER (probe logarithmic intensity error estimation). Glycome profiling was used to analyze cell wall composition in the samples. In addition, our results were compared to those of two other groups using the same hardware on the same mission (BRIC-16).

Key resul

SUBMITTER: Johnson CM 

PROVIDER: S-EPMC5821596 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature