Luminescent and paramagnetic properties of nanoparticles shed light on their interactions with proteins.

ABSTRACT: Nanoparticles have been recognized as promising tools for targeted drug-delivery and protein therapeutics. However, the mechanisms of protein-nanoparticle interaction and the dynamics underlying the binding process are poorly understood. Here, we present a general methodology for the characterization of protein-nanoparticle interaction on a molecular level. To this end we combined biophysical techniques including nuclear magnetic resonance (NMR), circular dichroism (CD), resonance energy transfer (RET) and surface plasmon resonance (SPR). Particularly, we analyzed molecular mechanisms and dynamics of the interaction of CaF2 nanoparticles with the prototypical calcium sensor calmodulin (CaM). We observed the transient formation of an intermediate encounter complex involving the structural r

SUBMITTER: Dal Cortivo G 

PROVIDER: S-EPMC5821874 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature