Nanoscale kinetic segregation of TCR and CD45 in engaged microvilli facilitates early T cell activation.

ABSTRACT: T cells have a central function in mounting immune responses. However, mechanisms of their early activation by cognate antigens remain incompletely understood. Here we use live-cell multi-colour single-molecule localization microscopy to study the dynamic separation between TCRs and CD45 glycoprotein phosphatases in early cell contacts under TCR-activating and non-activating conditions. Using atomic force microscopy, we identify these cell contacts with engaged microvilli and characterize their morphology, rigidity and dynamics. Physical modelling and simulations of the imaged cell interfaces quantitatively capture the TCR-CD45 separation. Surprisingly, TCR phosphorylation negatively correlates with TCR-CD45 separation. These data support a refined kinetic-segregation model. First, kinetic

SUBMITTER: Razvag Y 

PROVIDER: S-EPMC5821895 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature