Transcription start site profiling uncovers divergent transcription and enhancer-associated RNAs in Drosophila melanogaster.

ABSTRACT:

Background

High-resolution transcription start site (TSS) mapping in D. melanogaster embryos and cell lines has revealed a rich and detailed landscape of both cis- and trans-regulatory elements and factors. However, TSS profiling has not been investigated in an orthogonal in vivo setting. Here, we present a comprehensive dataset that links TSS dynamics with nucleosome occupancy and gene expression in the wandering third instar larva, a developmental stage characterized by large-scale shifts in transcriptional programs in preparation for metamorphosis.

Results

The data recapitulate major regulatory classes of TSSs, based on peak width, promoter-proximal polymerase pausing, and cis-regulatory element density. We confirm the paucity of divergent transcription units in D. melano

SUBMITTER: Meers MP 

PROVIDER: S-EPMC5822475 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature