Altered DNA methylation in liver and adipose tissues derived from individuals with obesity and type 2 diabetes.

ABSTRACT:

Background

Obesity is a well-recognized risk factor for insulin resistance and type 2 diabetes (T2D), although the precise mechanisms underlying the relationship remain unknown. In this study we identified alterations of DNA methylation influencing T2D pathogenesis, in subcutaneous and visceral adipose tissues, liver, and blood from individuals with obesity.

Methods

The study included individuals with obesity, with and without T2D. From these patients, we obtained samples of liver tissue (n = 16), visceral and subcutaneous adipose tissues (n = 30), and peripheral blood (n = 38). We analyzed DNA methylation using Illumina Infinium Human Methylation arrays, and gene expression profiles using HumanHT-12 Expression BeadChip Arrays.

Results

Analysis of DNA methylation prof

SUBMITTER: Barajas-Olmos F 

PROVIDER: S-EPMC5822594 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature