Genome-wide identification and analysis of A-to-I RNA editing events in bovine by transcriptome sequencing.

ABSTRACT: RNA editing increases the diversity of the transcriptome and proteome. Adenosine-to-inosine (A-to-I) editing is the predominant type of RNA editing in mammals and it is catalyzed by the adenosine deaminases acting on RNA (ADARs) family. Here, we used a largescale computational analysis of transcriptomic data from brain, heart, colon, lung, spleen, kidney, testes, skeletal muscle and liver, from three adult animals in order to identify RNA editing sites in bovine. We developed a computational pipeline and used a rigorous strategy to identify novel editing sites from RNA-Seq data in the absence of corresponding DNA sequence information. Our methods take into account sequencing errors, mapping bias, as well as biological replication to reduce the probability of obtaining a false-positive resu

SUBMITTER: Bakhtiarizadeh MR 

PROVIDER: S-EPMC5823453 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature