VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells.

ABSTRACT: T and NK cells armed with chimeric antigen receptors (CAR) are promising tools for the specific elimination of cancer cells. In most CAR designs implemented to date, the recognition of target cells is mediated by single-chain variable fragments (scFvs) derived from murine monoclonal antibodies. This format, however, has a number of limitations, including its relatively large size and potential immunogenicity in humans. In this study, we explored the feasibility of using human fibronectin type III domains (Fn3) as the antigen recognition domain in CARs. Human Fn3 domains have lower predicted immunogenicity compared to mouse-derived sequences, and a reduced molecular weight compared to scFvs. We created a functional CAR using a VEGFR2-specific Fn3 module replacing the conventional scFv. The

SUBMITTER: Kulemzin SV 

PROVIDER: S-EPMC5823625 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature