Analysis of a gene panel for targeted sequencing of colorectal cancer samples.

ABSTRACT: Colorectal cancer (CRC) is a leading cause of death worldwide. Surgical intervention is a successful treatment for stage I patients, whereas other more advanced cases may require adjuvant chemotherapy. The selection of effective adjuvant treatments remains, however, challenging. Accurate patient stratification is necessary for the identification of the subset of patients likely responding to treatment, while sparing others from pernicious treatment. Targeted sequencing approaches may help in this regard, enabling rapid genetic investigation, and at the same time easily applicable in routine diagnosis. We propose a set of guidelines for the identification, including variant calling and filtering, of somatic mutations driving tumorigenesis in the absence of matched healthy tissue. We also di

SUBMITTER: Jensen KH 

PROVIDER: S-EPMC5823670 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature