Elevation of autoantibody level against PDCD11 in patients with transient ischemic attack.

ABSTRACT:

Background

Disease specific autoantibodies have been detected in the sera of patients with atherosclerosis-related diseases, such as cerebral infarction, cardiovascular disease. In the present study, we aimed to identify novel autoantibodies responsible for transient ischemic attack (TIA), a prodromal condition for cerebral infarction.

Methods

To identify candidate antigens, we screened a human aortic endothelial cell cDNA library using sera from 20 patients with TIA. Serum antibody levels were measured using amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) in 2 independent patient/healthy donor (HD) cohorts (n = 192 and n = 906 in the second screening and validation cohort, respectively).

Results

First screening i

SUBMITTER: Yoshida Y 

PROVIDER: S-EPMC5823671 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature