Intervertebral Disc Degeneration in a Percutaneous Mouse Tail Injury Model.

ABSTRACT:

Objectives

Intervertebral disc (IVD) degenerates progressively with age and after injuries. In this study, we aimed to characterize early molecular events underlying disc degeneration using a mouse tail IVD injury model.

Design

We have established a transcutaneous minimally invasive approach to induce mouse tail IVD injury under fluoroscopic guidance. Morphological and molecular changes in the injured IVDs are compared with the baseline features of adjacent intact levels.

Results

After needle puncture, tail IVDs exhibited time-dependent histological changes. The aggrecan neoepitope VDIPEN was evident from 2 days to 4 wks after injury. A disintegrin and metalloproteinase domain-containing protein 8 (adam8) is a surface protease known to cleave fibronectin in the IVD. G

SUBMITTER: Tian Z 

PROVIDER: S-EPMC5823709 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature