TRPV4 (Transient Receptor Potential Vanilloid 4) Channel-Dependent Negative Feedback Mechanism Regulates G_q Protein-Coupled Receptor-Induced Vasoconstriction.

ABSTRACT:

Objective

Several physiological stimuli activate smooth muscle cell (SMC) G_qPCRs (G_q protein-coupled receptors) to cause vasoconstriction. As a protective mechanism against excessive vasoconstriction, SMC G_qPCR stimulation invokes endothelial cell vasodilatory signaling. Whether Ca²⁺ influx in endothelial cells contributes to the regulation of G_qPCR-induced vasoconstriction remains unknown. Ca²⁺ influx through TRPV4 (transient receptor potential vanilloid 4) channels is a key regulator of endothelium-dependent vasodilation. We hypothesized that SMC G_qPCR stimulation engages endothelial TRPV4 channels to limit vasoconstriction.

Approach and results

Using high-speed confocal microscopy to record unita

SUBMITTER: Hong K 

PROVIDER: S-EPMC5823749 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature