PPARα-Target Gene Expression Requires TIS21^/BTG2 Gene in Liver of the C57BL/6 Mice under Fasting Condition.

ABSTRACT: The TIS21^/BTG2/PC3 gene belongs to the antiproliferative gene (APRO) family and exhibits tumor suppressive activity. However, here we report that TIS21 controls lipid metabolism, rather than cell proliferation, under fasting condition. Using microarray analysis, whole gene expression changes were investigated in liver of TIS21 knockout (TIS21-KO) mice after 20 h fasting and compared with wild type (WT). Peroxisome proliferator-activated receptor alpha (PPARα) target gene expression was almost absent in contrast to increased lipid synthesis in the TIS21-KO mice compared to WT mice. Immunohistochemistry with hematoxylin and eosin staining revealed that lipid deposition was focal in the TIS21-KO liver as opposed to the diffuse and homogeneous pattern in the WT liver after 24 h star

SUBMITTER: Hong AE 

PROVIDER: S-EPMC5824024 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature