Mass Spectrometry-Based Screening Platform Reveals Orco Interactome in Drosophila melanogaster.

ABSTRACT: Animals use their odorant receptors to receive chemical information from the environment. Insect odorant receptors differ from the G protein-coupled odorant receptors in vertebrates and nematodes, and very little is known about their protein-protein interactions. Here, we introduce a mass spectrometric platform designed for the large-scale analysis of insect odorant receptor protein-protein interactions. Using this platform, we obtained the first Orco interactome from Drosophila melanogaster. From a total of 1,186 identified proteins, we narrowed the interaction candidates to 226, of which only two-thirds have been named. These candidates include the known olfactory proteins Or92a and Obp51a. Around 90% of the proteins having published names likely function inside the cell, and near

SUBMITTER: Yu KE 

PROVIDER: S-EPMC5824025 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature