Inhibition of MEK/ERK signalling pathway promotes erythroid differentiation and reduces HSCs engraftment in ex vivo expanded haematopoietic stem cells.

ABSTRACT: The MEK/ERK pathway is found to be important in regulating different biological processes such as proliferation, differentiation and survival in a wide variety of cells. However, its role in self-renewal of haematopoietic stem cells is controversial and remains to be clarified. The aim of this study was to understand the role of MEK/ERK pathway in ex vivo expansion of mononuclear cells (MNCs) and purified CD34⁺ cells, both derived from human umbilical cord blood (hUCB). Based on our results, culturing the cells in the presence of an inhibitor of MEK/ERK pathway-PD0325901 (PD)-significantly reduces the expansion of CD34⁺ and CD34⁺  CD38^- cells, while there is no change in the expression of stemness-related genes (HOXB4, BMI1). Moreover, in vivo an

SUBMITTER: Zarrabi M 

PROVIDER: S-EPMC5824365 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature