Leucocyte and Platelet-rich Fibrin: a carrier of autologous multipotent cells for regenerative medicine.

ABSTRACT: The wound healing is a complex process wherein inflammation, proliferation and regeneration evolve according to a spatio-temporal pattern from the activation of coagulation cascade to the formation of a plug clot including fibrin matrix, blood-borne cells and cytokines/growth factors. Creating environments conducive to tissue repair, the haemoderivatives are commonly proposed for the treatment of hard-to-heal wounds. Here, we explored in vitro the intrinsic regenerative potentialities of a leucocyte- and platelet-rich fibrin product, known as CPL-MB, defining the stemness grade of cells sprouting from the haemoderivative. Using highly concentrated serum-based medium to simulate wound conditions, we isolated fibroblast-like cells (CPL-CMCs) adhering to plastic and showing stable in vitro pr

SUBMITTER: Di Liddo R 

PROVIDER: S-EPMC5824368 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature