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Therapeutic benefits of CD90-negative cardiac stromal cells in rats with a 30-day chronic infarct.

ABSTRACT: Cardiac stromal cells (CSCs) can be derived from explant cultures, and a subgroup of these cells is viewed as cardiac mesenchymal stem cells due to their expression of CD90. Here, we sought to determine the therapeutic potential of CD90-positive and CD90-negative CSCs in a rat model of chronic myocardial infarction. We obtain CD90-positive and CD90-negative fractions of CSCs from rat myocardial tissue explant cultures by magnetically activated cell sorting. In vitro, CD90-negative CSCs outperform CD90-positive CSCs in tube formation and cardiomyocyte functional assays. In rats with a 30-day infarct, injection of CD90-negative CSCs augments cardiac function in the infarct in a way superior to that from CD90-positive CSCs and unsorted CSCs. Histological analysis revealed that CD90-negative CSCs increase vascularization in the infarct. Our results suggest that CD90-negative CSCs could be a development candidate as a new cell therapy product for chronic myocardial infarction.

SUBMITTER: Shen D

PROVIDER: S-EPMC5824400 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Therapeutic benefits of CD90-negative cardiac stromal cells in rats with a 30-day chronic infarct.

Shen Deliang D Shen Miaoda M Liang Hongxia H Tang Junnan J Wang Bo B Liu Chuang C Wang Peiwen P Dong Jianzeng J Li Ling L Zhang Jinying J Caranasos Thomas G TG

Journal of cellular and molecular medicine 20180117 3

Cardiac stromal cells (CSCs) can be derived from explant cultures, and a subgroup of these cells is viewed as cardiac mesenchymal stem cells due to their expression of CD90. Here, we sought to determine the therapeutic potential of CD90-positive and CD90-negative CSCs in a rat model of chronic myocardial infarction. We obtain CD90-positive and CD90-negative fractions of CSCs from rat myocardial tissue explant cultures by magnetically activated cell sorting. In vitro, CD90-negative CSCs outperfor ...[more]

PMID: 29341439

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Therapeutic benefits of CD90-negative cardiac stromal cells in rats with a 30-day chronic infarct.

Publications

Therapeutic benefits of CD90-negative cardiac stromal cells in rats with a 30-day chronic infarct.

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